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The yeast UASG is a transcriptional enhancer in human hela cells in the presence of the GAL4 trans -activator

Identifieur interne : 000407 ( France/Analysis ); précédent : 000406; suivant : 000408

The yeast UASG is a transcriptional enhancer in human hela cells in the presence of the GAL4 trans -activator

Auteurs : Nicholas Webster ; Jia Rui Jin [France] ; Stephen Green [France] ; Melvyn Hollis [États-Unis] ; Pierre Chambon [France]

Source :

RBID : ISTEX:F93AD5F4E6BECF17113A3C523918F4C3B276E675

Descripteurs français

English descriptors

Abstract

Abstract: The yeast trans-activator protein GAL4, when expressed in HeLa cells, stimulates transcription from several class B (II) eukaryotic promoters containing GAL4 binding sites either as the full UASG or as synthetic 17-mers. The characteristics of this activation are indistinguishable from those of the SV40 enhancer. Transcription was similarly stimulated from either complex promoter regions containing multiple upstream elements or from a simple promoter region composed of only a TATA box. Addition of a 17-mer GAL4 binding site to the SV40 enhancer resulted in a synergistic enhancement of transcription in the presence of GAL4. Furthermore, chimeras of the human estrogen receptor DNA binding domain and either GAL4 or GCN4 activating “acidic” regions can activate a promoter region controlled by an estrogen-responsive enhancer. Together, these data indicate that the molecular mechanisms responsible for transcriptional enhancement have been conserved from yeast to man.

Url:
DOI: 10.1016/0092-8674(88)90505-3


Affiliations:


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ISTEX:F93AD5F4E6BECF17113A3C523918F4C3B276E675

Le document en format XML

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<term>Estrogens (pharmacology)</term>
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<term>Plasmids</term>
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<term>Rabbits</term>
<term>Receptors, Estrogen (genetics)</term>
<term>Regulatory Sequences, Nucleic Acid</term>
<term>Saccharomyces cerevisiae Proteins</term>
<term>Simian virus 40 (genetics)</term>
<term>Simplexvirus (enzymology)</term>
<term>Simplexvirus (genetics)</term>
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<term>Oestrogènes (pharmacologie)</term>
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<term>Protéines de Saccharomyces cerevisiae</term>
<term>Protéines de liaison à l'ADN</term>
<term>Protéines fongiques (génétique)</term>
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<term>Simplexvirus (génétique)</term>
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<term>Transfection</term>
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<term>Acidic</term>
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<term>Activator</term>
<term>Amino</term>
<term>Amino acid core</term>
<term>Amino acids</term>
<term>Animals</term>
<term>Binding domain</term>
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<term>Rabbits</term>
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<term>Reference gene</term>
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<div type="abstract" xml:lang="en">Abstract: The yeast trans-activator protein GAL4, when expressed in HeLa cells, stimulates transcription from several class B (II) eukaryotic promoters containing GAL4 binding sites either as the full UASG or as synthetic 17-mers. The characteristics of this activation are indistinguishable from those of the SV40 enhancer. Transcription was similarly stimulated from either complex promoter regions containing multiple upstream elements or from a simple promoter region composed of only a TATA box. Addition of a 17-mer GAL4 binding site to the SV40 enhancer resulted in a synergistic enhancement of transcription in the presence of GAL4. Furthermore, chimeras of the human estrogen receptor DNA binding domain and either GAL4 or GCN4 activating “acidic” regions can activate a promoter region controlled by an estrogen-responsive enhancer. Together, these data indicate that the molecular mechanisms responsible for transcriptional enhancement have been conserved from yeast to man.</div>
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